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IVF Step-by-Step Guide: What to Expect During the IVF Procedure

IVF Step-by-Step Guide: What to Expect During the IVF Procedure

IVF Procedure – IVF journey can feel overwhelming, but knowing what happens at each stage can help ease your mind. IVF (in-vitro fertilisation) is a fertility treatment where eggs and sperm are combined in a laboratory to help achieve pregnancy.

This guide gives you a simple breakdown of the IVF process step by step.

IVF may seem like a step-by-step checklist, but each stage is highly detailed. From preparing the entire reproductive system, to ovarian stimulation and finally to embryo replacement, every step is critical in improving the chances of success

Prof Ng Soon ChyeMedical Chairman, Senior Fertility Specialist
IVF Procedure - Step-by-step Guide

The IVF Procedure – Step-by-Step Guide


Step 1: Initial Consultation

Step 1 of the IVF procedure is the initial consultation. Your IVF journey begins with a consultation with a fertility specialist.

  • You and your husband’s medical history will be reviewed.
  • Review of your fertility screening test results.
  • Discussion about the goals of treatment

👉 This stage helps the fertility specialist tailor a treatment plan that suits you.


Step 2: Counselling Session

This involves financial counselling and explanation of the IVF process including possible side effects due to the hormone injections.

👉 The purpose is to help you plan your finances and set your expectations.


Step 3: Ovarian Stimulation

Normally, women ovulate just one egg per month. With IVF, you’ll take daily hormone injections for 8–12 days to produce multiple eggs.

  • Regular ultrasounds and blood tests will monitor your response.
  • Your doctor may adjust your medication to keep everything safe and effective.

The nurse will provide guidance on how to inject your medication.

👉 Think of this step as “encouraging your ovaries to grow more eggs.”


Step 4: Trigger Shot

When your follicles grow to a good size, you’ll be given a “trigger injection” (usually hCG) to help your eggs mature.

  • The timing is important — it’s usually given 36 hours before egg retrieval.


Step 5: Egg and Sperm Retrieval

Egg retrieval is a short procedure, done under sedation so you won’t feel pain.

  • A thin needle is guided by ultrasound to collect as many of your eggs as possible.
  • You may feel mild cramping afterward, but recovery is quick.

Fresh semen sample will be collected from the husband on the same day. Alternatively, a frozen sperm sample stored before your IVF cycle may also be used.

👉 This is a day surgery procedure. You’ll be monitored in the recovery room for 1-2 hours after the procedure.


Step 6: Fertilisation and Embryo Development in the Lab

The collected eggs are combined with sperm in the IVF laboratory.

  • ICSI (Intracytoplasmic Sperm Injection): A single sperm is injected directly into an egg – a standard procedure that our embryologists will do.
  • The fertilised eggs (embryos) will be cultured in a controlled environment (special incubator) and monitored using a time-lapse monitoring system to observe the daily development and progress to ensure they are growing under optimal conditions.
  • Once the embryos reach the blastocysts stage, you may undergo fresh embryo transfer if your doctor assessed you to be suitable. Otherwise, the embryologists typically freeze the embryos for your future use in a frozen-thawed embryo transfer cycle.

👉 Embryologists carefully monitor this process which typically can take 6 to 7 days.


Step 7: Preparation for Embryo Transfer

You’ll be advised when to start medications in preparation for the Embryo Transfer. Typically, it will be from Day 3 of menses and continue for 16 days.

  • You’ll be required to do ultrasounds and blood tests on Day 10 and Day 14.
  • Fertility specialist will monitor the thickness of your uterine lining and hormone levels to ensure timing is perfect.

👉 Once your lining is ready, a date is fixed for the embryo transfer.


Step 8: Embryo Transfer

On the day of transfer, your chosen frozen embryo is carefully thawed in the laboratory by embryologists.

  • Assisted Hatching: another standard procedure done in our lab to assist in the natural hatching process and help increase the likelihood of successful implantation.

Embryo transfer is a simple, painless procedure.

  • A thin catheter places the embryo into your uterus.
  • Sedation is not needed. You can usually go home soon after.

👉 The procedure feels like a pap smear for most patients.


Step 9: The Two-Week Wait

After transfer, you’ll take medications (such as progesterone) to support implantation.

  • This waiting period can feel stressful, but it’s an important part of the process.
  • Try to rest, eat well, and lean on your support system.


Step 10: Pregnancy Test

About 10–14 days after embryo transfer, you’ll take a blood test to confirm pregnancy.

  • If positive, your clinic will guide you with early pregnancy care.
  • If negative, your doctor will discuss the next steps and possible adjustments for future cycles.


Final Thoughts On The IVF Procedure

IVF is a journey of hope and courage. While not every cycle leads to pregnancy, each step brings you closer to your goal. Your fertility team will be with you at every stage, providing guidance and support.

💙 Remember: Every IVF journey is unique. Don’t hesitate to ask your fertility specialist questions and take care of yourself — physically and emotionally — along the way.

IVF FAQs

1: How long does the IVF process take?

From ovarian stimulation to embryo transfer, a single IVF cycle usually takes 4–6 weeks.

2: Is IVF painful?

Most steps are not painful. You may feel mild discomfort from injections or cramping after egg retrieval, but pain relief and sedation make it manageable.

3: What are my chances of success?

Success rates depend on age, fertility health, and other factors. Your fertility specialist will explain your chances based on your individual situation.

4: Can embryos be frozen for later use?

Yes, many patients freeze embryos for planned frozen-thawed embryo transfer as well future attempts, especially if there are extra good-quality embryos.

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